Research on donkey milk · Study 85

5 % donkey milk in the cheese vat: how 80 tasters judged

A team from the University of Basilicata made a caciotta from cow’s milk – once plain, once with 5 % donkey milk. After 10 and 45 days of ripening, 80 cheese consumers rated colour, aroma, taste, texture and overall impression.

Important: A consumer test on sensory properties – no statement about any effect in humans. What was rated was a cow’s milk cheese with 5 % added donkey milk, not a pure donkey milk cheese.
Study typeConsumer test (sensory) with cheese analysis
Participants80 adults (41 men, 39 women), 21–58 years
CheeseCaciotta from cow’s milk, plain or with 5 % donkey milk; ripened 10 and 45 days
OriginUniversity of Basilicata, Potenza, Italy · 2022

The starting milk

Cow’s and donkey milk compared

Cow’s milk (Friesian cows)Donkey milk (Martina Franca donkeys)

Figures in per cent, means of duplicate determinations. Donkey milk contained less fat and protein but more milk sugar. Because only 5 % donkey milk was added, the finished cheeses did not differ statistically in dry matter, protein, fat and ash – but they did in pH.

Production · runs automatically

From the vat to the ripening shelf

The cow’s milk was pasteurised for 30 minutes at 65 °C; according to the paper, the donkey milk was not heated. 100 litres of cow’s milk per vat; the whole trial was carried out twice. Wheels shown schematically.

Key results · choose an attribute

Nine levels of liking

    Each level is one point on the 9-point scale (1 = dislike extremely, 5 = neither like nor dislike, 9 = like extremely). The markers sit at the means from Table 2 and are placed to scale between the levels. Standard deviations are around 0.9 to 1.5 points.

    Critical assessment

    Our checklist for the tasting test

    Checklist · Study 85

    ✓
    Blind tasting

    Samples with random three-digit codes, in random order, in individual booths. The participants did not know the experimental design.

    ~
    Who tasted?

    80 habitual cheese eaters, recruited via notices at the university – not a representative sample.

    Whether the same people rated all four cheeses is not stated explicitly.
    ✗
    Text and table don’t quite match

    The text calls the aroma difference after 10 days “significant (p < 0.05)”, while Table 2 gives p = 0.051.

    ~
    Statistics

    According to the paper, the interaction of cheese type and ripening time was not significant – even though the ranking reversed between 10 and 45 days. How the two trial runs were factored in remains open.

    ~
    Scope

    A short paper (“Communication”), described by the authors themselves as preliminary. A trained expert panel and an aroma compound analysis are missing.

    ✗
    Not examined

    Shelf life, antimicrobial or probiotic properties, which the authors mention in the discussion, were not measured.

    We do not adopt these assumptions.

    Funding & interests

    Funded by the rural development programme of the Basilicata region (project INNOPROLATTE). The authors declare no conflicts of interest; according to the paper, the funder had no influence.

    Level of evidence

    Sensory consumer test in humans – describes taste preferences, no statement about effects.

    Research question for DON DONKEY’S®

    How does ripening time change the taste of cheese with donkey milk?

    In this study, the verdict reversed between 10 and 45 days. We would like to understand better how the aroma and texture of donkey milk cheese develop with ripening – as a question of taste, not of health.

    Compare with Study 68Document ripeningDescribe the flavour profile

    Original publication

    Effect of the Addition of Donkey Milk on the Acceptability of Caciotta Cow Cheese

    Original title of the publication.

    Cosentino C., Paolino R., Rubino M., Freschi P.
    Animals · 2022 · volume 12, issue 11, article 1444 · open access (short communication)
    School of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Potenza (Italy)

    DOI: 10.3390/ani12111444

    Transparency: funded by the Basilicata region (project INNOPROLATTE), no conflicts of interest according to the paper. Consent of all participants was obtained. No product-related statement for DON DONKEY’S®.

    Summarised and reviewed by the DON DONKEY’S editorial team on the basis of the original publication · as of October 2026

    End of the study summary
    Note on our products

    The study summarised above was carried out independently of DON DONKEY’S® and does not relate to our products. It serves solely to inform about the state of research.

    DON DONKEY’S® donkey cheese and donkey milk are foods. They are not intended to prevent, treat or cure diseases and do not replace medical advice.

    DON DONKEY&rsquo;S&reg; donkey cheese

    From our shop

    DON DONKEY’S® donkey cheese

    Cheese nuggets from 50 g

    Matching the topic of this page: our donkey cheese. Around 25 litres of donkey milk go into one kilogram; the taste is slightly nutty, the texture firm and creamy at the same time.

    Transparency: The study made a caciotta from cow’s milk with 5 % donkey milk – not our donkey cheese. The results cannot be transferred to our product.
    €249,90

    50 grams · Unit price: €4.998,00 / 1 kg · incl. VAT, plus shipping

    View all sizes & purchase options →
    DON DONKEY&rsquo;S&reg; donkey milk

    Also in our shop

    DON DONKEY’S® donkey milk

    100 % donkey milk · raw milk, frozen

    On this page we present the findings of a published study. Independently of that, you will find our donkey milk here: 100 % donkey milk, frozen as raw milk.

    Transparency: In the study, the donkey milk came from Martina Franca jennies from southern Italy – not from our partner farms.
    €24,90

    100 ml bottle / 100ml · Unit price: €249,00 / 1 l · incl. VAT, plus shipping

    View all sizes & purchase options →