Research on donkey milk · Study 6

Donkey and cow's milk in a laboratory test

In 2020, Daminelli et al. compared how two well-known foodborne bacteria – Listeria and Staphylococcus aureus – develop in raw donkey milk and raw cow's milk under defined laboratory conditions.

Important: The milk samples were deliberately inoculated with bacteria in the laboratory and then observed. This is a model experiment, not an examination of commercially available milk.
Publication
Daminelli et al.
2020 · Veterinaria Italiana
Study type
Preliminary laboratory study
(challenge test)
Conditions
11 days
at 8 °C
Focus
Listeria &
Staphylococcus aureus

What it's about

A question of food safety

Donkey milk contains a lot of lysozyme and other components to which antibacterial properties are attributed. Researchers from the IZSLER institute in Brescia and the University of Bari tested whether this results in a difference from cow's milk in practice.

In the laboratory, they inoculated raw donkey milk and raw cow's milk with Listeria monocytogenes and Staphylococcus aureus and followed how the bacteria developed over eleven days. They also measured lactic acid bacteria and the pH value.

The result turned out differently than one might expect given the lysozyme – and that is exactly why it belongs on this page.

What exactly was studied?

MilkRaw donkey milk and raw cow's milk
BacteriaListeria and Staphylococcus aureus, deliberately added
MeasuredBacterial count, lactic acid bacteria, pH value
Not studiedCommercial milk, consumption, illness
ResultListeria grew more in donkey milk
LimitationPreliminary study under laboratory conditions

Briefly explained

The terms used in this study

MethodChallenge test

A food is deliberately inoculated with bacteria in the laboratory to observe whether and how strongly they multiply in it.

BacteriaListeria

Listeria monocytogenes is a bacterium that can grow even at refrigerator temperature and receives particular attention in food safety.

BacteriaLactic acid bacteria

Bacteria naturally occurring in milk that produce lactic acid and thereby lower the pH value.

Measurementlog10 CFU/ml

Bacterial counts are given logarithmically: one step more means ten times as many bacteria.

Step 01Preparing

Raw donkey milk and raw cow's milk were provided as test samples.

Step 02Inoculate

The samples were deliberately inoculated with Listeria or Staphylococcus aureus.

Step 03Store

The samples were kept for eleven days at 8 °C – a defined test condition.

Step 04Measure

Bacterial counts, lactic acid bacteria and pH value were determined over time.

Interactive · The data at a glance

Eleven days in the laboratory

How was the experiment set up, how did the Listeria develop – and what was found for Staphylococcus aureus, lactic acid bacteria and pH value? Switch between the views.

2types of milk: raw from the donkey, raw from the cow
2bacteria deliberately added: Listeria, S. aureus
8 °Cdefined storage temperature in the experiment
11days of observation with repeated measurements

Illustration of the mean values reported in the original publication. Lines connect the start and end values and do not represent a measured course.

Key findings

What the researchers report

Listeria

Stronger growth in donkey milk

+2.63log10 · donkey milk
+0.95log10 · cow's milk
Day 11: 6.31 vs. 4.59 log10 CFU/ml

With the same starting amount, the Listeria count after eleven days was arithmetically about 50 times higher in raw donkey milk than in raw cow's milk.

Staphylococcus aureus

No significant difference

≈Donkey milk
≈Cow's milk
both types of milk comparable

For Staphylococcus aureus, the authors report no statistically significant difference between the two types of milk.

Milk flora & pH

Fewer lactic acid bacteria, higher pH

fewerlactic acid bacteria
higherpH value
possible explanation according to the authors

Donkey milk contained fewer lactic acid bacteria and had a higher pH value. The authors see this as a possible explanation for the stronger growth of Listeria.

The study is described as preliminary. The milk was deliberately inoculated with bacteria and stored under fixed conditions (11 days, 8 °C). The final value for cow's milk varied widely (± 1.04 log10). The CFU comparisons are converted from the published mean values. Whether lactic acid bacteria or pH value actually caused the difference was not demonstrated.

Critical assessment

An honest result – even if it doesn't favour donkey milk

What the study shows

✓
Under the test conditions, Listeria grew more in raw donkey milk than in raw cow's milk.
✓
For Staphylococcus aureus there was no significant difference.
✓
Lysozyme and other components did not prevent the growth of Listeria here.
✓
The milk flora and pH value of the two types of milk differed clearly.

What it does not show

×
No statement on how often Listeria actually occur in donkey milk.
×
No statement on illness after consumption.
×
No general safety ranking of the two types of milk.
×
No transferability to other temperatures, batches or processing methods.

Research question for DON DONKEY’S®

What does this mean for our work?

The study shows: the quality of donkey milk does not depend on its natural components alone, but on care during milking, chilling and processing. That is the standard we set for our own milk.

01 · MILKINGCareful processes from the animal to bottling.
02 · COLD CHAINPrompt chilling and freezing without interruption.
03 · LAB CHECKSHave our milk analysed regularly.
04 · TRANSPARENCYAlso showing studies that don't favour donkey milk.

Original publication

Raw donkey milk versus raw cow’s milk. A preliminary study to compare the growth of Listeria monocytogenes and Staphylococcus aureus

Daminelli P., Dalzini E., Cosciani-Cunico E., Barrasso R., Bozzo G., Zanardi G.
Veterinaria Italiana · 2020 · 56(2):115–121
IZSLER Brescia, University of Bari Aldo Moro

DOI: 10.12834/VetIt.2140.13666.1 · PubMed: PMID 33305560
Full text: PDF of the original publication

The publication describes a laboratory experiment with deliberately inoculated milk. It does not allow any product-related statement for DON DONKEY’S®.

DON DONKEY’S® donkey milk

From our shop

DON DONKEY’S® donkey milk

100 % donkey milk · raw milk, frozen

On this page we report the findings of a published study. Independently of that, you will find our donkey milk here: 100 % donkey milk, frozen as raw milk.

Transparency: In the study, milk was deliberately inoculated with bacteria in the laboratory, not DON DONKEY’S® donkey milk. Our donkey milk is a food.
€24,90

100 ml bottle / 100ml · Unit price: €249,00 / 1 l · incl. VAT, plus shipping

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Donkey Milk Powder

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Donkey Milk Powder

Donkey milk in powder form

For anyone who prefers a longer shelf life: our donkey milk in powder form – easy to store and portion.

Transparency: In the study, milk was deliberately inoculated with bacteria in the laboratory, not our donkey milk powder. Our donkey milk powder is a food.
€24,90

20g · Unit price: €1.245,00 / 1 kg · incl. VAT, plus shipping

View all sizes & purchase options →